Subgroup analysis was further performed by dividing the anti-CENP-B antibody positive group into the solitary anti-CENP-B antibody positive along with additional auto-antibodies positive organizations to determine the characters related to anti-CENP-B antibody. == Results == In this study, 288 individuals with pSS were evaluated, including 75 individuals with anti-CENP-B antibody positive and 213 with anti-CENP-B antibody negative. Aceglutamide positive and negative groups. Intergroup variations were analyzed with SPSS 23.0 software. Subgroup analysis was further performed by dividing the anti-CENP-B antibody positive group into the solitary anti-CENP-B antibody positive along with additional auto-antibodies positive organizations Aceglutamide to determine the characters related Aceglutamide to anti-CENP-B antibody. == Results == Aceglutamide With this study, 288 individuals with pSS were evaluated, including 75 individuals with anti-CENP-B antibody positive and 213 with anti-CENP-B antibody bad. Univariate analysis showed that compared with the anti-CENP-B antibody bad group, the individuals of the anti-CENP-B antibody positive group were older, experienced lower proportion of the individuals with salivary gland enlargement and higher proportion of autoimmune liver disease. As for immunological signals, the positive proportions of anti-SSA/Ro60, anti-Ro52, and anti-SSB antibodies were significantly lower. Moreover, the immunoglobulin (Ig) G and rheumatoid element levels were significantly lower, while the IgM level was significantly higher in the individuals of the anti-CENP-B antibody positive group. As for serum biochemical signals, for the individuals of the anti-CENP-B antibody positive group, the level of total protein (TP) was lower, the albumin/globulin percentage was higher, and the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), lactate dehydrogenase (LDH) were higher. Subgroup analysis showed the levels of TP and IgA in the individuals of the solitary anti-CENP-B antibody positive group were significantly lower than those of the individuals with additional autoantibodies positive group. == Summary == The pSS individuals with anti-CENP-B antibody positive have unique medical and immunological features of lower disease activity, less likely to involve salivary gland, higher risk for autoimmune liver disease, and higher levels of liver function signals. Anti-CENP-B antibody may be a marker for a distinct subset of polyautoimmunity in Sjgren’s syndrome. Keywords:Centromere protein B antibody, Main Sjgren’s syndrome, Autoantibodies, Immuno-logical features (main Sjgrens syndrome, pSS)(Raynaud trend)[1] pSS71%pSSSSA/Ro6067%pSSSSB[2]SSA/Ro602016(American College of Rheumatology, ACR)/(Western Little league Against Rheumatism, EULAR)pSS[3]pSSpSS B(centromere protein B, CENP-B)DNACENP-B(anti-centromere antibody, ACA)[4-5]CENP-B(systemic sclerosis, SSc)[6]ACApSS8.2%~25.3%[7-8]CENP-BpSSSSA/Ro60SSBCENP-BpSS[9]CENP-BpSSpSSCENP-BpSS == 1. == == 1.1. == 2016120228pSS(1)2016ACR/EULARpSS4[3](2)18(1)pSSIgG4(2)pSS(3)pSS(4) CENP-BpSSCENP-BCENP-BCENP-B (PKUSSIIT2022062)(PKUSSIRB-202281144) == 1.2. == pSS(Schirmer)2016ACR/EULARpSS[3]0.1 mL/min5 mm/5 min5 pSS140 mmHg90 mmHg[10-12] == 1.3. == [13-14]pSS011 mm21~2 mm3>2 mm401~4 4 mm2123( 4) == 1.4. == 15(immunoglobulin, Ig)3(match, C3)(rheumatoid element, RF) (EUROLINEANA Profile 3EUROMMUNLbeck)nRNP/SmSmSSA/Ro60Ro52SSBScl-70PM-SclJo-1CENP-B(proliferating cell nuclear antigenPCNA)PM2(anti-mitochondrial antibody M2, AMA-M2)(double-stranded deoxyribonucleic aciddsDNA)EUROLineScanIgGIgAIgMC3()RF() (total protein, TP)(65.0~85.0 g/L)(albumin, Alb)(40.0~55.0 g/L)/(albumin/globulin, A/G)(1.20~2.40)(alanine aminotransferase, ALT)(7~40 U/L)(aspartate aminotransferase, AST)(13~35 U/L)AST/ALT(0.8~1.5)(total bilirubin, TBIL)(3.42~20.50 mol/L)(direct bilirubin, DBIL)(0.00~6.84 mol/L)(alkaline phosphatase, ALP)(50~135 U/L)-(gamma glutamyl transferase, GGT)(7~45 U/L)(creatine kinase, CK)(24~140 U/L)(lactate AMPK dehydroge-nase, LDH)(109~245 U/L)(International Federation of Clinical Chemistry and Laboratory Medicine, IFCC) == 1.5. == SPSS 23.0=0.05P< 0.05xs2FishertMann-WhitneyCENP-BLogistic < 10%10%~30%>30% == 2. == == 2.1. Aceglutamide == 2016120228pSS1 5032016ACR/EULARpSSpSS646IgG4267pSS15514766576CENP-BpSS288( 1) == 1. == pSS The circulation diagram of screening and grouping of pSS individuals pSS, main Sjgren’s syndrome; SGE, salivary gland enlargement; ACR, American College of Rheumatology; EULAR, Western Little league Against Rheumatism; CENP-B, centromere protein B. == 2.2. == 288pSSCENP-B75213pSS(274, 95.1%)CENP-B(P>0.05)CENP-BCENP-B[63.0 (54.0, 71.0)vs.53.0 (38.5, 62.0),P< 0.05]CENP-B[22 (29.3%)vs.98 (46.0%),P< 0.05](P>0.05)>30% 1CENP-B4/mm2(P>0.05)5pSSCENP-B4112CENP-B1CENP-B[4, (5.3%)vs.1 (0.5%),P< 0.05] 1 == 1. == CENP-BpSS Assessment of the medical features of the anti-CENP-B antibody positive and negative pSS individuals == 2.3. == == 2.3.1. == CENP-B14CENP-BSSA/Ro60Ro52SSBCENP-B[36 (48.0%)vs.190 (89.2%)39 (52.0%)vs.178 (83.6%)10 (13.3%)vs.108 (50.7%)P< 0.05]CENP-B[6 (8.0%)vs.3 (1.4%),P< 0.05]CENP-BIgGRF[17.90 (13.51, 19.12) g/Lvs.19.55 (16.24, 24.73) g/L18.20 (5.55, 30.42) IU/mLvs.30.47 (19.30, 105.89) IU/mL,P< 0.05]IgM[1.34 (1.07, 2.42) g/Lvs.1.34 (0.92, 1.65) g/L,P< 0.05] 2LogisticCENP-BIgG(OR=0.897, 95%CI: 0.850~0.947)IgM(OR=1.321, 95%CI: 1.079~ 1.618) 3 == 2. == Assessment of autoantibodies along with other immune signals between organizations == 3. == CENP-B Multivariate analysis of anti-CENP-B antibody and immunoglobulins by binary Logistic.