For total VDJ sequencing of Ig large stores, cells were sorted into 96 well plates containing lysis buffer (PBS containing 3U/l RNAsin, 10mM dithiothreitol DTT).
For total VDJ sequencing of Ig large stores, cells were sorted into 96 well plates containing lysis buffer (PBS containing 3U/l RNAsin, 10mM dithiothreitol DTT).